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| description |
The purpose of this study was to develop and apply a quantitative
optical method suitable for routine measurements of biofilm
structures under in situ conditions. A computer program was designed
to perform automated investigations of biofilms by using image
acquisition and image analysis techniques. To obtain a
representative profile of a growing biofilm, a nondestructive
procedure was created to study and quantify undisturbed microbial
populations within the physical environment of a glass flow cell.
Key components of the computer-controlled processing described in
this paper are the on-line collection of confocal two-dimensional
(2D) cross-sectional images from a preset 3D domain of interest
followed by the off-line analysis of these 2D images. With the
quantitative extraction of information contained in each image, a
three-dimensional reconstruction of the principal biological events
can be achieved. The program is convenient to handle and was
generated to determine biovolumes and thus facilitate the
examination of dynamic processes within biofilms. In the present
study, Pseudomonas fluorescens or a green fluorescent
protein-expressing Escherichia coli strain, EC12, was inoculated
into glass flow cells and the respective monoculture biofilms were
analyzed in three dimensions. In this paper we describe a method for
the routine measurements of biofilms by using automated image
acquisition and semiautomated image analysis.
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| publisher |
The American Society for Microbiology
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| type |
Text
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| Article in Journal
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| source |
In: Journal of Applied and Environmental Microbiology. Vol. 64(11),
pp. 4115-4127
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| contributor |
IPVS, Simulation großer Systeme
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| Hans-Joachim Bungartz
| subject |
Simulation and Modeling (CR I.6)
| | Life and Medical Sciences (CR J.3)
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